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drosofiller  (Genesee Scientific)


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    Structured Review

    Genesee Scientific drosofiller
    Drosofiller, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/droso+filler/Droso-filler%2C+Narrow+Vials/pmc11872617-419-8-13
    Average 90 stars, based on 2 article reviews
    drosofiller - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    In Vivo:

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Photo-conversion filter cube list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) Software list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 ImageJ ( https://imagej.nih.gov/ij/ ) Video to video converter ( http://www.videotovideo.org/ ) Zeiss Zen Blue Lite ( https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html ) Procedure A. ..

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay Photo-conversion filter cube 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) .. Brush (Craft Smart ® round brush set golden taklon) (Michaels Stores, model: Size 3, catalog number: 10408282) Nikon body plus lens combination (Nikon, model: D5300) and AF-S Nikkor 18–55 mm DX VRII (Nikon, model: AF-S DX) Tripod for mounting DSLR Cold plate cooler (TE Technology, model: CP-031) Cold plate temperature controller (TE Technology, model: TC-720) Cold plate power supply (TE Technology, model: PS-12-8.4A) Infrared thermometer (Fluke, model: Fluke 62 MAX) Aluminum plate–Laminated aluminum shim (Global Equipment, catalog number: WBB512969) Notes: Cut the shim to 7.5 by 11.5 mm at 0.22 mm thickness.

    Microscopy:

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Photo-conversion filter cube list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) Software list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 ImageJ ( https://imagej.nih.gov/ij/ ) Video to video converter ( http://www.videotovideo.org/ ) Zeiss Zen Blue Lite ( https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html ) Procedure A. ..

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay Photo-conversion filter cube 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) .. Brush (Craft Smart ® round brush set golden taklon) (Michaels Stores, model: Size 3, catalog number: 10408282) Nikon body plus lens combination (Nikon, model: D5300) and AF-S Nikkor 18–55 mm DX VRII (Nikon, model: AF-S DX) Tripod for mounting DSLR Cold plate cooler (TE Technology, model: CP-031) Cold plate temperature controller (TE Technology, model: TC-720) Cold plate power supply (TE Technology, model: PS-12-8.4A) Infrared thermometer (Fluke, model: Fluke 62 MAX) Aluminum plate–Laminated aluminum shim (Global Equipment, catalog number: WBB512969) Notes: Cut the shim to 7.5 by 11.5 mm at 0.22 mm thickness.

    Imaging:

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Photo-conversion filter cube list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) Software list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 ImageJ ( https://imagej.nih.gov/ij/ ) Video to video converter ( http://www.videotovideo.org/ ) Zeiss Zen Blue Lite ( https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html ) Procedure A. ..

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay Photo-conversion filter cube 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) .. Brush (Craft Smart ® round brush set golden taklon) (Michaels Stores, model: Size 3, catalog number: 10408282) Nikon body plus lens combination (Nikon, model: D5300) and AF-S Nikkor 18–55 mm DX VRII (Nikon, model: AF-S DX) Tripod for mounting DSLR Cold plate cooler (TE Technology, model: CP-031) Cold plate temperature controller (TE Technology, model: TC-720) Cold plate power supply (TE Technology, model: PS-12-8.4A) Infrared thermometer (Fluke, model: Fluke 62 MAX) Aluminum plate–Laminated aluminum shim (Global Equipment, catalog number: WBB512969) Notes: Cut the shim to 7.5 by 11.5 mm at 0.22 mm thickness.

    Transferring:

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Photo-conversion filter cube list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) Software list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 ImageJ ( https://imagej.nih.gov/ij/ ) Video to video converter ( http://www.videotovideo.org/ ) Zeiss Zen Blue Lite ( https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html ) Procedure A. ..

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Photo-conversion filter cube 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay .. Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) .. ImageJ ( https://imagej.nih.gov/ij/ ) Video to video converter ( http://www.videotovideo.org/ ) Zeiss Zen Blue Lite ( https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html )

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay Photo-conversion filter cube 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) .. Brush (Craft Smart ® round brush set golden taklon) (Michaels Stores, model: Size 3, catalog number: 10408282) Nikon body plus lens combination (Nikon, model: D5300) and AF-S Nikkor 18–55 mm DX VRII (Nikon, model: AF-S DX) Tripod for mounting DSLR Cold plate cooler (TE Technology, model: CP-031) Cold plate temperature controller (TE Technology, model: TC-720) Cold plate power supply (TE Technology, model: PS-12-8.4A) Infrared thermometer (Fluke, model: Fluke 62 MAX) Aluminum plate–Laminated aluminum shim (Global Equipment, catalog number: WBB512969) Notes: Cut the shim to 7.5 by 11.5 mm at 0.22 mm thickness.

    Software:

    Article Title: Behavioral and Functional Assays for Investigating Mechanisms of Noxious Cold Detection and Multimodal Sensory Processing in Drosophila Larvae
    Article Snippet: Black spray paint–12 oz. black flat general purpose spray paint (Rust-Oleum) B. .. In vivo GCaMP assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 PE120 Peltier stage (Linkam Scientific Instruments, Linkam Scientific, model: PE120) T95 system controller (including T95 linkpad and PE95) from (Linkam Scientific Instruments, Linkam Scientific, model: T95) Laser confocal microscope capable of imaging GFP (Carl Zeiss, model: LSM 780) C. Optogenetic dose response assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 D5300 DSLR (Nikon, model: D5300) Adapters for mounting Nikon DSLR onto Zeiss microscopes: T2-adapter for Nikon F (Carl Zeiss, model: T2-adapter, catalog number: 416009-0000-000) and Adapter 60N–T2 1.0x (Carl Zeiss, model: Adapter 60N, catalog number: 426103-0000-000) Glass plate: 10 x 15 x 0.1 cm D. CaMPARI Ca 2+ integrator assay list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Photo-conversion filter cube list-behavior=enumerated prefix-word= mark-type=lower-alpha max-label-size=0 612/69 BrightLine bandpass filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-612/69-25) 440 nm BrightLine SWP edge filter, 25 mm (IDEX Health & Science, Semrock, catalog number: FF01-440/SP-25) 562 BrightLine dichroic beamsplitter, 25.2 x 35.6 mm (IDEX Health & Science, Semrock, catalog number: FF562-Di03-25x36) Axio Zoom.V16 (ZEISS, model: Axio Zoom.V16) with Illuminator HXP200c lamp (Carl Zeiss, model: HXP 200C) Light touch stimulus: Nickel plated pin holder (Fine Science Tools, catalog number: 26018-17) with mounted single fine paint brush bristle Noxious cold stimulus: TE technology cold plate cooler described in cold plate assay E. Drosophila media preparation list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Adventurer Pro II Analytical/Precision Balance (Ohaus, model: AX2202) FastPette V2 Pipette Controller (Labnet International, catalog number: P2000) 10ml Serological Pipets (Genesee Scientific, catalog number: 12-104) Droso-Filler, Narrow (Genesee Scientific, catalog number: 59-168) Avantco Induction Range (Avantco Equipment, model: IC3500) Stainless Stock Pot with lid (Thunder Group, catalog number: SLSPS020) Narrow Fly Vial Reload Tray (Genesee Scientific, catalog number: 59-207) Software list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 ImageJ ( https://imagej.nih.gov/ij/ ) Video to video converter ( http://www.videotovideo.org/ ) Zeiss Zen Blue Lite ( https://www.zeiss.com/microscopy/us/products/microscope-software/zen-lite.html ) Procedure A. ..



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    90
    Genesee Scientific drosofiller
    Drosofiller, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    drosofiller - by Bioz Stars, 2026-09
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    Genesee Scientific drosofillertm
    Drosofillertm, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    drosofillertm - by Bioz Stars, 2026-09
    90/100 stars
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    Genesee Scientific droso-filler, narrow 59-168
    Droso Filler, Narrow 59 168, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    droso-filler, narrow 59-168 - by Bioz Stars, 2026-09
    90/100 stars
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    Genesee Scientific droso filler
    Droso Filler, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/droso+filler/Droso-filler%2C+Narrow+Vials/pmc05564679-42-243-245
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    droso filler - by Bioz Stars, 2026-09
    90/100 stars
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    Genesee Scientific food pourer
    Food Pourer, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/droso+filler/Droso-filler%2C+Wide+Vials/10__1038_slash_nprot__2007__404-88-12-14
    Average 90 stars, based on 1 article reviews
    food pourer - by Bioz Stars, 2026-09
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